Fig. 3. Co-localization of endogenous vezatin and the myosin VIIA tail fused to GFP in transfected MDCK cells. As soon as cell contacts can be detected, the myosin VIIA tail (A) co-localizes with endogenous vezatin (B) at the precise membrane sites of the cell–cell contacts (C). The same result was obtained with a GFP–myosin VIIA tail fragment composed of the last 464 amino acids of myosin VIIA (not shown). After Triton X-100 treatment the GFP–myosin VIIA tail (D) is still associated with the actin complex (E) at the cell–cell junctions (F). Bar, 10 µm.