Fig. 1. Reinitiation in SeqA-deficient cells. Cells of strain CM742 (A) or CM742seqA (ALO1417; B) were grown exponentially at 30°C. At time T = –90, cultures were shifted to 42°C, kept at this non-permissive temperature for 90 min and at time T = 0 shifted back to 30°C. At the times indicated, samples of the cultures were taken and treated with rifampicin and cephalexin for 4 h prior to flow cytometric analysis. The numbers of origins per cell were determined as described in Materials and methods. The small panels on the right hand side of the figure show selected histograms.