Fig. 6. Nucleoid distribution in SeqA-deficient cells. Exponentially growing ALO1266 (wt) or ALO1393 (ΔseqA) cells were incubated for 15 min with 300 µg/ml chloramphenicol to condense the nucleoids. In order to form filaments, cells were incubated with 20 µg/ml cephalexin for approximately three mass doublings prior to condensing the nucleoids. Cells were fixed and stained prior to microscopic analysis as described in Materials and methods.