Skip to main content
. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Nat Neurosci. 2010 Dec 5;14(1):22–24. doi: 10.1038/nn.2703

Figure 1.

Figure 1

Transient and targeted attenuation of striatal cell signaling. (a, h) Amplicon maps of pENK-hM4D/pENK-GFP (a) and pDYN-hM4D/pDYN-GFP (h) targeting vectors. (b, i) Confocal microscopy showed that pENK-hM4D receptors were selectively expressed in striatopallidal MSNs (b) whereas pDYN-hM4D receptors were selectively expressed in striatonigral MSNs (i). Green, hemagglutinin (HA); Red, ENK (top) and substance P (SP, bottom); Yellow, co-localization of neurons. Scale bars, 10 μm. (c) Representative voltage trace of CNO-induced hyperpolarization of an hM4D-expressing striatal neuron. (d, e) CNO decreased input resistance in hM4D-expressing neurons. * P < 0.05 hM4D before vs. hM4D after CNO application, n=4–5. (f, g) Representative traces (f) and summarized data (g) showed that CNO decreased the number of evoked action potentials in hM4D-expressing neurons. ** P < 0.01 hM4D vs. hM4D/CNO (k, n) CNO-mediated activation of pENK-hM4D (k) or pDYN-hM4D (n) receptors decreased the number of amphetamine-induced Fos cells (CON: vehicle-treated pENK-hM4D and pDYN-hM4D, respectively) (pENK: P = 0.002, n=5–6/group; pDYN: P < 0.05, n=5–6/group). (l, o) Amphetamine-evoked c-Fos+ cells were reduced in both hemagglutinin-positive (P < 0.05) and hemagglutinin-negative (P < 0.01) neurons in the pENK-hM4D experiment (l) and in hemagglutinin-positive neurons (P < 0.05) in the pDYN-hM4D experiment (o). Representative Fos immunohistochemistry sections (red) from pENK-hM4D (j) and pDYN-hM4D (m) infused striatum of vehicle (VEH) and CNO-treated rats. Scale bars, 50 μm. Insets depict single-labeled Fos cells (red), hemagglutinin cells (green) and dual-labeled cells (yellow). Scale bars, 10 μm. Data represent mean ± SEM. V = vehicle treatment, C = CNO treatment.