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. 2000 Nov 15;19(22):6196–6206. doi: 10.1093/emboj/19.22.6196

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graphic file with name cdd603f2a.jpg — Fig. 2. Leptomycin B inhibits STAT1 nuclear export. (A) U3A cells expressing STAT1–GFP were pre-treated for 30 min with cyclo heximide (all panels) and leptomycin B (LMB) (right panels) and then pulse-treated with IFN-γ for 30 min. The cellular localization of STAT1–GFP was visualized by fluorescent microscopy. Time after IFN-γ treatment is indicated to the left of the panels. (B) Cells from each treatment condition in (A) were collected and immunoprecipitated with anti-STAT1 antibody. Western blots were performed with anti-STAT1 phosphotyrosine antibody (anti-STAT1 pY) (upper panel) or anti-STAT1 antibody (lower panel).

graphic file with name cdd603f2b.jpg — Fig. 2. Leptomycin B inhibits STAT1 nuclear export. (A) U3A cells expressing STAT1–GFP were pre-treated for 30 min with cyclo heximide (all panels) and leptomycin B (LMB) (right panels) and then pulse-treated with IFN-γ for 30 min. The cellular localization of STAT1–GFP was visualized by fluorescent microscopy. Time after IFN-γ treatment is indicated to the left of the panels. (B) Cells from each treatment condition in (A) were collected and immunoprecipitated with anti-STAT1 antibody. Western blots were performed with anti-STAT1 phosphotyrosine antibody (anti-STAT1 pY) (upper panel) or anti-STAT1 antibody (lower panel).