Skip to main content
. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Cancer Res. 2010 Nov 30;70(23):9937–9948. doi: 10.1158/0008-5472.CAN-10-0881

Figure 5. Notch signaling maintains ALDH+ lung cancer cells and sensitized ALDH+ cells to Notch signaling inhibition.

Figure 5

A. Expression of Notch signaling transcript (shown as an expression ration in ALDH+/ALDH− cells) is elevated in ALDH+ lung cancer cells. B. qRT-PCR analysis revealed a decrease in Notch signaling transcription factors in unsorted lung cancer cells treated with 10 µM DAPT for 24hrs. C. Cell lines treated with 10 µM DAPT for five days retained significantly smaller ALDH+ populations compared to DMSO treated cells (n = 3, *P < 0.05). D. Sorted H358 ALDH+ and ALDH− cells were treated with DMSO and DAPT and cell cycle analysis was performed revealing a greater accumulation of cells in G1/G0-phase as well as a decrease of cells in S-phase in DAPT treated ALDH+ cells compared to DAPT treated ALDH− cells.