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. Author manuscript; available in PMC: 2011 Dec 1.

Published in final edited form as: Anal Chem. 2010 Nov 1;82(23):9694–9701. doi: 10.1021/ac101714u

(A) Surface immobilized GQ-DNA molecules encapsulated inside either eggPC () or eggPC/Chol (■) vesicles (10 mM Tris, 50 mM KCl, pH 8.0) were incubated with various concentrations of aHL at the room temperature. After 60 min incubation, excess aHL was flushed with 0 mM K⁺ buffer and the percentage of unfolded population (U) was quantified from the histogram. The relative unfolded populations are plotted against aHL concentrations and fitted with Hill equation (with Hill coefficient = 1, solid lines). The fit of the data yielded 490±90 nM and 14±3 nM for eggPC and 1:2 eggPC:Chol vesicle, respectively. The permeable vesicles reached saturation at close to 90% beyond 0.74 μM. (B) In the similar manner, various incubation times were tested with 0.74 μM aHL. The single-exponential fit of the data yielded rate constant of 2.1±0.2 min.

Inline graphic — (A) Surface immobilized GQ-DNA molecules encapsulated inside either eggPC () or eggPC/Chol (■) vesicles (10 mM Tris, 50 mM KCl, pH 8.0) were incubated with various concentrations of aHL at the room temperature. After 60 min incubation, excess aHL was flushed with 0 mM K⁺ buffer and the percentage of unfolded population (U) was quantified from the histogram. The relative unfolded populations are plotted against aHL concentrations and fitted with Hill equation (with Hill coefficient = 1, solid lines). The fit of the data yielded 490±90 nM and 14±3 nM for eggPC and 1:2 eggPC:Chol vesicle, respectively. The permeable vesicles reached saturation at close to 90% beyond 0.74 μM. (B) In the similar manner, various incubation times were tested with 0.74 μM aHL. The single-exponential fit of the data yielded rate constant of 2.1±0.2 min.