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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Anal Chem. 2010 Nov 1;82(23):9694–9701. doi: 10.1021/ac101714u

Figure 3. Successive Buffer Exchange.

Figure 3

Time trace of donor and acceptor intensities and corresponding E from a single molecule starting at 0 mM K+ condition and successive addition of buffers containing 2 mM and 10 mM K+ are shown above (0.5 sec integration time). The trace shows fluctuations between the unfolded (mid E state) and two folded states (high E states).