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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Anal Chem. 2010 Nov 1;82(23):9694–9701. doi: 10.1021/ac101714u

Figure 5. Comparison between aHL pores and thermotropic DMPC pores.

Figure 5

smFRET histograms above demonstrate the temperature independent porousity of aHL pores. GQ-DNA strands were encapsulated in eggPC/Chol and DMPC vesicles with 50 mM K+. Without pores, potassium ions will remain within the vesicle leaving GQ-DNA molecules in two folded states (high E populations) even after flushing with 0 mM K+ Tris buffer. At all temperatures, GQ-DNA in vesicles with aHL pores show a large shift to the unfolded state (mid E), on the contrary, GQ-DNA in DMPC showed a similar shift most efficiently when the temperature was close to the Tm of DMPC (23 °C). No shift to the unfolded state was observed at 17 °C and a delayed transition to the unfolded state was observed at 37 °C for DMPC system. The data for 37 °C was taken within 2 min of the buffer exchange.