Cy3 (donor) labeled Rep helicase and Cy5 (acceptor) labeled partial duplex DNA have been co-encapsulated within eggPC/Chol vesicle. Data taken at 16, 22, 30 and 33 °C are shown above. From these time traces, translocation period, Δt, was measured and plotted as histograms. Average Δt’s from Gaussian fits of these histograms are shown on the plot.