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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Curr Protoc Toxicol. 2011 Feb;CHAPTER:Unit22.2. doi: 10.1002/0471140856.tx2202s47

Figure 1. Side population profile in human clinical prostate specimen (NT1) based upon DCV efflux.

Figure 1

Live cells are gated based on 7AAD staining and Forward Scatter (FSC) vs Side Scatter (SSC) plot (A and D) and singlets are plotted (B and E). The gated side population cells are displayed in a double scatter plot between Red 650 nm and Blue (450/40 nm) emission (C). ABCG2 mediated DCV efflux is inhibited in the presence of FTC (D-F). The side population profile of the specimen used in this experiment is 0.9% (C) which is inhibited in the presence of FTC (F).
  • FSC – Forward Scatter
  • SSC – Side Scatter
  • FSC-H – Forward Scatter Height
  • FSC-W – Forward Scatter Width
  • SP – Side Population
  • NSP – Non-side Population