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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Nat Struct Mol Biol. 2010 Dec 5;18(1):27–34. doi: 10.1038/nsmb.1934

Figure 6.

Figure 6

Correlation between transcripts from two alleles of a constitutively active gene, MDN1, in diploid cells. (a) Schematic diagram of the PP7 array inserted in the 3′ untranslated region of one of the two endogenous MDN1 alleles. (b) Transcripts from both alleles were detected with cyanine 3 labeled probes hybridizing to the coding region of MDN1 (green). Transcripts from Allele 2 (yellow) were distinguished with colocalizing signals from cyanine 3.5 labeled probes against 11 binding sites in the 24x PP7 array (red). The scale bar in the DIC image is 1 μm. (c) Heat map of number of transcripts from two MDN1 alleles in 217 diploid cells. The correlation coefficient (r) between transcripts from two alleles in the same cell was 0.33±0.04. The distribution of mRNAs per cell for each allele across the population is depicted by the marginal histograms: μMDN1 = 6.6±0.3, σMDN1 = 4.0 (top); μMDN1-PP7 = 4.8±0.3, σMDN1-PP7 = 2.8 (right). Error bars indicate s.e.m.