Figure 2. Phasic dopamine signaling in response to CS and US presentation during the acquisition of Pavlovian conditioned approach behavior in bHR and bLR rats.
Phasic dopamine release was recorded in the core of the nucleus accumbens using FSCV across six days of training. (a, d) Representative surface plots depict trial-by-trial fluctuations in dopamine concentration during the twenty-second period around CS and US presentation in individual animals throughout training. (b, e) Mean + SEM change in dopamine concentration in response to CS and US presentation for each session of conditioning. (c, f) Mean + SEM change in peak amplitude of the dopamine signal observed in response to CS and US presentation for each session of conditioning (n=5/group; Bonferroni post-hoc comparison between CS- and US-evoked dopamine release: *P<0.05; **P<0.01). Panels a-c demonstrate that bHR animals, which developed a sign-tracking CR, show increasing phasic dopamine responses to CS presentation and decreasing responses to US presentation across the six sessions of training. In contrast, panels d-f demonstrate that bLR rats, which developed a goal-tracking CR, maintain phasic responses to US presentation throughout training.