Table 3.

TLR9 relative gene expression.

Sample	TLR9*	IFN-alpha1*	IRF7*
PBMC	0.024	0.67	0.08
PBMC depleted from pDCs	0.015	0.40	0.077
pDCs	3.73	2.83	4.60

^*TLR9 gene expression was compared in freshly isolated PBMC and pDCs by RT-qPCR. IFN-alpha1 and IRF-7 gene expression were tested in the same samples. The human GAPDH, housekeeping gene, was used to normalize gene expression in different samples. The results are representative of three different experiments done with three different healthy subjects.