Table 2.
Molecular identification, relative abundance, observed total and mean species richness (± SE) and estimated species richness of ectomycorrhizal fungi on the roots of naturally regenerating seedlings (n = 12 at each site) and mature trees (n = 20 soil samples at each site) of P. mugo grown on coastal dunes of Curonian Spit
| Identification | Closest match | Identity (%) | Relative abundance (%) | |||||
|---|---|---|---|---|---|---|---|---|
| S1 | S2 | S3 | ||||||
| Seedlings | Mature trees | Seedlings | Mature trees | Seedlings | Mature trees | |||
| Ascomycota | ||||||||
| Cenococcum geophilum | Cenococcum geophilum (DQ068980) | 99 | 62.0 ± 4.16aa | 31.0 ± 2.44b | 52.0 ± 4.99a | 29.2 ± 3.95b | 56.8 ± 6.56a | 34.1 + 3.09b |
| Wilcoxina sp. | Wilcoxina rehmii (DQ069001) | 97 | 18.8 ± 2.81A | 26.7 ± 3.89 | 27.8 ± 3.05A | 32.8 ± 3.88 | 7.7 ± 2.40Bb | 29.7 + 3.73a |
| Pezizomycotina | uncultured Pezizomycotina (DQ273331) | 90 | 2.8 ± 2.08AB | 3.3 ± 1.64 | 0.5 ± 0.47B | 5.3 ± 2.52 | 15.5 ± 4.66A | 5.2 + 3.43 |
| Meliniomyces sp. | Meliniomyces variabilis (EF093173) | 97 | 0.1 ± 0.06 | 0.5 ± 0.37 | – | 0.5 ± 0.35 | – | – |
| Basidiomycota | ||||||||
| Russula paludosa | Russula paludosa (AJ971402) | 98 | 8.9 ± 3.21 | 13.6 ± 4.04 | 8.8 ± 1.92 | 6.5 ± 3.25 | 16.0 ± 3.00 | 8.5 + 2.04 |
| Suilloid | Suillus bovinus (L54077) | 99 | 5.5 ± 2.56 | 5.9 ± 1.97 | 4.5 ± 1.78 | 2.4 ± 1.69 | 1.1 ± 0.72 | 5.8 + 1.78 |
| Suillus luteus (GU373495) | ||||||||
| Basidiomycota | Uncultured ectomycorrhiza (FJ348387) | 90 | 0.8 ± 0.84 | – | 0.6 ± 0.61 | 0.4 ± 0.37 | 0.4 ± 0.45 | – |
| Lactarius rufus | Lactarius rufus (EU346873) | 99 | 0.4 ± 0.33b | 18.3 ± 4.63a | 5.5 ± 3.68 | 21.3 ± 5.40 | 2.6 ± 1.39b | 10.8 + 2.23a |
| Cortinarius sp. | Cortinarius sp. (AF430288) | 96 | 0.4 ± 0.39 | 0.6 ± 0.47 | – | 1.2 ± 0.71 | – | 2.5 + 1.38 |
| Cortinarius obtusus | Cortinarius cf. obtusus (FJ039610) | 98 | 0.3 ± 0.29 | – | 0.1 ± 0.14 | – | – | 3.1 + 1.43 |
| Cortinarius croceus | Cortinarius croceus (UDB000129) | 99 | – | – | 0.1 ± 0.14 | 0.4 ± 0.37 | – | 0.3 + 0.31 |
| Coexistent taxa | ||||||||
| Unidentified | Uncultured fungal clone (EU292463) | 96 | + | + | + | |||
| Helotiales | Uncultured Helotiales (EF619698) | 98 | + | + | + | |||
| Observed species richness | 11 | 9 | 10 | 11 | 8 | 10 | ||
| Mean no species per seedling/soil sample | 4.2 ± 0.27 | 3.6 ± 0.18Bb | 4.1 ± 0.34 | 3.3 ± 0.19B | 4.2 ± 0.24 | 4.5 + 0.20A | ||
| Jackknife 1 | 14.67 | 9.28 | 13.67 | 12.90 | 8.92 | 10.95 | ||
| Jackknife 2 | 17.24 | 9.31 | 17.00 | 12.38 | 8.23 | 11.85 | ||
| Bootstrap | 12.55 | 9.25 | 11.42 | 12.14 | 8.58 | 10.45 | ||
| Shannon diversity index (H′) | 0.98 ± 0.08 | 1.18 ± 0.05 | 1.09 ± 0.08 | 1.05 ± 0.06 | 1.01 ± 0.08 | 1.31 ± 0.06 | ||
aDifferent letters indicate significant differences at site level (a) and between examined sites (A) at a P value of <0.05 (Mann–Whitney U test)
bDifferent letters indicate significant differences at site level (a) and between examined sites (A) according to the t test and Tukey’s (HSD) test (P < 0.05), respectively