Table II. Novel CDC5L complex proteins and other proteins that although present in the complex have not previously been shown to be associated with the spliceosome.
| Protein name | SwissProt/DDBJ/EMBL/GenBank accession No. | Mol. wt (kDa) | Orthologues (yeast) | Comments/motifs |
|---|---|---|---|---|
| TOP IIα | Q9UP44 | 175.1 | TOP2p | TOP I activates SR proteins by phosphorylation and interacts with PSF/p54nrb. |
| PP2c (WIP1) | O15297 | 67.4 | PTC1 | Induced in response to ionizing radiation (Fiscella et al., 1997). |
| DDP kinase | Q13327 | 470.4 | TOR1p | |
| PP1-α | P08129 | 38.2 | GLC7 | Very high sequence conservation (93% homology between yeast and human). |
| NF45 | Q12905 | 44.9 | ||
| CCAP1* (HS7C) | P11142 | 71.1 | Ssa2p | 75% homology to Drosophila protein (TrEMBL Q9VG73). |
| CCAP2 (HSPC 148) | AAF29112 (DDBJ/EMBL/GenBank) | 26.6 | SPBC337.06c | Putative RNA helicase with DExD motif; has ATP/GTP-binding site motif A (P-loop). |
| CCAP3* | Q9UMS4 | 55.6 | PRP19-like | Contains several WD40a sequence motifs. |
| CCAP4 | Q15394 | 49 | Has leucine zipper pattern DNA binding motif; ATP/GTP-binding site (P-loop) motif A. | |
| CCAP5 | Q9Y4G5 | 239.9 | Contains several putative PP1-bindingb domain sequence motifs; ATP/GTP binding site motif (P-loop); leucine zipper pattern. | |
| CCAP6* | Q92616 | 266.3 | Has RNP-1 and DNA binding motifs (leucine zipper pattern); putative histidine acid phosphatase. | |
| CCAP7 | Q9UPN6 | 140.8 | Has SR repeat motif domain; ATP/GTP binding site (P-loop) and RNP-1 sequence motifs. | |
| CCAP8 | O60289 | 145.4 | Contains RNA binding and two WWc domain motifs. |
*Core protein in the CDC5L-associated protein complex.
aReviewed by Smith et al. (1999).
cEspanel and Sudol (1999) and references therein.