Table I. Average secondary structure content (%) of wild-type RAP46 and the multiple serine-substituted mutant (RAP46mutSer) as calculated from the far-UV CD spectra (see Materials and methods).

Secondary structure estimation	Wild-type RAP46	RAP46mtSer
α-helix	21	34
β-sheet	27	9
Turn	9	32
Polyproline helix	15	15
Other structures	8	19