Figure 2. Biliverdin and bilirubin suppression of HCV replication.

Log-phase full-length (FL) (A) or non-structural (NS) (B) replicon cells were treated 48 hours with indicated concentrations of BV or BR. HCV replication was determined by Real-Time RT-PCR using the Comparative Cycle Threshold (ΔC_T) method as described in Supplemental methods available on line. Each value is the mean +/− the SEM of 5–6 determinations per treatment group. Biliverdin = >99% Biliverdin IX-α. Bilirubin-mixed isomers (MI) = 93% Bilirubin IX-α, and 6% associated Bilirubin isomers as described in Materials. Bilirubin IX-α = >99% Bilirubin IX-α.

[** or ## or ^^ p<0.01 from respective controls. ^ p < 0.05 from control.].