Fig. 9. Intragenic suppression of cold-sensitive growth. prp22Δ cells carrying wild-type PRP22, S635A, or the indicated intragenic suppressors were grown in liquid medium at 37°C. The A₆₀₀ was adjusted to 0.1. Aliquots (3 µl) of serial 10-fold dilutions were spotted onto YPD agar medium. The plates were photographed after incubation for 3 days at 37 and 30°C, or 4 days at 25°C.