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. 2010 Jan 20;44(4):225–236. doi: 10.1677/JME-09-0161

Figure 3.

Figure 3

PKA, p38 MAPK, JNK, and IKK are involved in α-MSH-induced Il6 gene expression and secretion. Differentiated 3T3-L1 adipocytes were pretreated with A, SB203580 (10 μM); B, SP600125 (10 μM); C, Bay 11-7085 (10 μM); and D, H89 (1 μM) and 6-Bnz-cAMP (50 μM) for 30 min prior to treatment with α-MSH (300 nM) for 1 h. (D) 3T3-L1 adipocytes were also treated with forskolin (3 μM), and isoproterenol (3 μM) alone for 1 h. (E) In the presence of each inhibitor, cells were treated with α-MSH for indicated time intervals, and culture medium was collected to measure secreted IL6. Data represent the means±s.d. of triplicate samples. *P<0·05; **P<0·001 compared with the α-MSH-treated group. #P<0·001 compared with the control group. Each experiment was performed independently at least three times.