Fig. 5. Alignment of Trbp111-like domains. Fifty-two sequences were aligned with A.aeolicus Trbp111. The naming convention is as follows: the first letter indicates family (T, Trbp111-like; M, C-term of MetRS; A, Arc1p-like; E, EmapII-like; Y, C-term of TyrRS); the second letter indicates kingdom (E, eukaryotes; B, eubacteria; A, archaea). The species name then follows (up to 10 characters total). Conservation levels are indicated by shading, as follows: 100% similarity across all species is shown in red; 80% similarity is shown in green; blue denotes 60% similarity. The locations of mutants in Trbp111 having no effect on tRNA binding are indicated with blue triangles and those locations identified as important for tRNA binding are indicated with red triangles.