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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Nature. 2010 Jul 29;466(7306):642–646. doi: 10.1038/nature09190

Fig. 3. Verification of EEF2 TASRs using independent biochemical methods (A–C) and possible mechanism for their production using endogenous RdRP-like activity (D).

Fig. 3

(A) Probes used in Northern blot and RPA assays. Regions of no complementarity in the probes are shown as thin oblique lines. (B) Northern blot results. The arrowhead marks the strongest consistent band. (C) RPA assays results. The arrowhead marks the expected protected fragment. (D) Schematic representation of 5’ polyU and 3’ polyA sRNAs flanking a gene locus. The existence of RNAs with both a 5' polyU and a 3' polyA sequence has not been demonstrated by these experiments and is thus hypothetical.