Fig. 1. Alignment and sequence elements of the five yeast proteins of the Puf RNA-binding family. Linear representations of Puf proteins 1–5 are drawn to scale, with the characteristic Puf repeat regions (denoted as eight black vertical rectangles) aligned within each protein. Puf1p and Puf2p also contain putative RRM RNA-binding domains (gray boxes), while Puf3p and Puf4p contain putative zinc finger domains (box labeled Zn). A C-terminal sequence region related in Puf2p and Puf5p is denoted by boxes labeled XXXX.