Fig. 6. puf3Δ does not affect the polysome distribution of COX17 mRNA. Shown are polysome analyses examining the distribution of the COX17 transcript in fractions along a polysome gradient from a wild-type (WT) strain (A) and a puf3Δ strain (B). The OD tracings of the gradients (measured as OD₂₅₄) are shown on the right of each panel. Northern blots of the RNA in each fraction are shown to the left of the polysome profile from which they were taken. The total RNA lane represents an aliquot of RNA from the cytoplasmic extract prior to loading on sucrose gradients.