Fig. 2. Intracytoplasmic expression of p34 and p58. Confocal immunofluorescence microscopy of HEp-2 cells transfected with DNA encoding GFP–p34 (A), GFP–p58 (C) or GFP–VacA (E). Expressed proteins were stained with antibodies directed against p34 (B) and the C-terminal part of VacA (D and F). Arrows indicate the presence of non-transfected cells not reacting with specific antibodies. Bar, 10 µm.