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. 2011 Mar 14;208(3):439–454. doi: 10.1084/jem.20101907

Figure 2.

Figure 2.

Reversibility of HIV neutralization. (A and B) Reversibility of neutralization was probed as depicted in the flow chart. Antibody concentrations were chosen to yield neutralization activities of ∼90% after 11 h of preincubation to allow for monitoring decreases in neutralization activity after the dissociation step. JR-FL neutralization by 0.1 µg/ml b12, 15 µg/ml 2G12, 11.4 µg/ml 2F5, and 45 µg/ml 4E10 was probed under the indicated treatment conditions: after antibody dissociation (treatment A), neutralization without dissociation (treatment B), and without removal of antibody (treatment C). Means and SEM of triplicates measurements of one of three independent experiments are shown. Abs, antibodies.