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. 2011 Mar 14;208(3):439–454. doi: 10.1084/jem.20101907

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© 2011 Ruprecht et al.

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Figure 6. — Shedding and neutralization activity of MPER mAbs follow identical kinetics and thermodynamic requirements. (A) To compare the kinetics of shedding and neutralization, neutralization on TZM-bl cells and shedding activity (as described in Fig. 3 B) of JR-FL pseudovirus upon treatment with mAbs b12 (0.03 µg/ml), 2G12 (12 µg/ml), 2F5 (2.5 µg/ml), and 4E10 (8.5 µg/ml) was assessed at the indicated times at 37°C. Chosen concentrations correspond to ∼70% neutralization after 20-h incubation at 37°C. One of two independent experiments is depicted. (B) To compare thermodynamic requirements of shedding and neutralization, neutralization on TZM-bl cells and shedding activity (as described in Fig. 3 B) of JR-FL pseudovirus upon treatment with mAbs b12 (0.03 µg/ml), 2G12 (12 µg/ml), 2F5 (2.5 µg/ml), and 4E10 (8.5 µg/ml) for 20 h at the indicated temperature were assessed. One of three independent experiments is depicted. (A and B) Chosen concentrations correspond to ∼70% neutralization after 20-h incubation at 37°C.