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. 2011 Mar 14;208(3):593–604. doi: 10.1084/jem.20100793

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© 2011 Barrett et al.

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Figure 5. — BMC^GM-CSF LTC₄S is required for Df-elicited CD4⁺ Th2 cell recruitment to the lung. WT mice were sensitized with saline-pulsed or Df-pulsed WT or Ltc4s^−/− BMCs^GM-CSF and challenged with Df as described in the legend to Fig. 4. Pulmonary mononuclear cells were isolated, stimulated with 50 ng/ml PMA and 1 µM ionomycin for 10 h in the presence of 2.5 µM monensin, stained for cell surface expression of CD4 and CD8 (A) and for intracellular expression of IL-4, IL-5, IL-17A, and IFN-γ (B), and analyzed by flow cytometry. Gating on cell size, on CD4 and CD8 expression, the total number of cells recruited to the lung is shown. Results are means ± SEM (n = 11 mice per group) from two experiments. *, P = 0.02; **, P = 0.005; ***, P = 0.002; ****, P = 0.0008. Significance was determined with an unpaired Student’s t test.