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. 2011 Mar 14;208(3):593–604. doi: 10.1084/jem.20100793

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© 2011 Barrett et al.

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Figure 8. — Df-elicited Th2 cytokine generation is dependent on BMC^GM-CSF CysLT₁R. WT mice were sensitized with saline-pulsed or Df-pulsed WT or Cysltr1^−/− BMCs^GM-CSF and challenged with Df as described in the legend to Fig. 7. Parabronchial lymph node cells were isolated, counted, and restimulated for 72 h with 0, 1, or 5 µg/ml Df, and cytokines in the supernatant were measured by ELISA. Total cytokine production per mouse is shown. Results are means ± SEM (n = 7 mice per group) from two experiments. Triangles under x axes indicate 0, 1, and 5 µg/ml Df, from left to right. *, P = 0.03; **, P = 0.02. Significance was determined with an unpaired Student’s t test.