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. 2010 Dec 15;202(12):1920–1929. doi: 10.1086/657414

Figure 3.

Figure 3.

A, Northern blot analysis of total RNA from Pneumocystis carinii. The hybridization signal of ∼1.2 kb (indicated by the arrow) was detected when the blot was hybridized with digoxigenin-labeled oligonucleotide GK1dmc1 (complementary to nucleotides 786-822 of P. carinii dmc1 complementary DNA). B, Southern blot analysis of genomic DNA from P. carinii. Genomic DNA extracted from partially purified P. carinii organisms was digested with AseI or EcoRI. Digoxigenin-labeled oligonucleotide GK1 was used as the probe for hybridization. DNA digested with AseI (lane 1) or EcoRI (lane 2) shows a single band, indicating that dmc1 is a single-copy gene. C, Western blot analysis of protein samples from Pneumocystis. Protein samples prepared from partially purified P. carinii or Pneumocystis murina-infected or uninfected mouse lung were subjected to Western blot analysis with the anti-Dmc1 antibody. Both P. murina (lane 2) and P. carinii (lane 3) preparations showed immunoreactivity to the ∼48-kDa band (indicated by the arrow). The uninfected mouse lung preparation (lane 1) showed no similar immunoreactivity.