Figure 5.

Exogenous heparin rescues ligand-receptor binding function of HSPGs disrupted by NaClO₃ treatment. A) Phase images illustrating that 1 μg/ml heparin was able to revert colony morphology of mESCs treated with NaClO₃ for 72 h to that of control cells cultured in SC medium. B) Gene expression of mESCs after 72 h perfusion at varying shear stresses with mESC-CM + 20 mM NaClO₃ and 1 μg/ml heparin. Inset: R² and whether regression slope differs significantly from 0. C) Comparison between expression of Fgf5 (i), Nanog (ii), Rex1 (iii), and Oct4 (iv) in mESC-CM perfusion cultures with NaClO₃ alone or NaClO₃ + heparin. Data are averages ± se of 3 independent experiments. *P < 0.05.