Figure 4.

Effect of PAF inhibition on retinal and choroidal P-selectin up-regulation. To quantify the level of P-selectin expression in retinal and choroidal endothelium of normal and EIU rats, with and without PAF inhibition, we injected rPSGL-1-conjugated MSs, 24 h after LPS injection, and performed in vivo molecular imaging of the fundus vessels or ex vivo epifluorescence microscopy of retinal and choroidal flat mounts. A) In vivo binding analysis in choroidal vessels was performed 30 min after MS injection. B) Quantification of MS binding to choroidal microvessels in normal and EIU rats with or without inhibitor pretreatment. C) Representative retinal flat mounts from normal and EIU animals with or without inhibitor treatments. Bright green spots, adhering MSs; round red cells in vessels, firmly adhering leukocytes. D) Quantification of the number of accumulated rPSGL-1-conjugated MSs in the retinal vessels of normal and EIU animals with or without PAF inhibitor treatment. E) Representative choroidal flat mounts from normal and EIU animals with or without inhibitor treatments. F) Quantification of the number of accumulated rPSGL-1-conjugated MSs in the choroidal vessels of normal and EIU animals with or without PAF inhibitor treatment. Bars are means ± se.