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. 2010 Nov 30;90(1):105–112. doi: 10.1093/cvr/cvq379

Figure 4.

Figure 4

ICaT blockade decreases PP2A-induced CREB dephosphorylation. (A) Effect of PP2A and PP2B on CREB phosphorylation. Total proteins were extracted from cells exposed for 24 h to 1 µM FK506 (FK) or 5 nM okadaic acid (OA). n = 3 performed in duplicate. (B) Aldosterone increases PP2A–PP1 activities via ICaT signalling. Total PP2A and PP1 activities were quantified in cells treated for 24 h with 1 µM aldosterone and 1 µM mibefradil or 5 µM fluoxetine (AM, AFluo) or ICaT and ICaL blockers (AMN); 12 nM OA blocked PP2A activity and 5 µM OA blocked PP2A and PP1 activities. Protein phosphatase inhibitor-2 (I-P1) blocked PP1 activity. PhosPep = 0.1 mM phosphopeptide alone. n = 3 performed in triplicate. *P < 0.05.