Figure 2.

Rac1 and Rho A differentially regulate Ao gene expression. CFs were transfected with adenoviruses expressing GFP alone (control) or coexpressed with dominant-negative forms of Rac1 (Rac1-DN) or RhoA (RhoA-DN). After 24 h of viral transfection, viral medium was replaced with fresh serum-free medium for stretch experiment. (A–C) Detection of morphological alterations after different viral treatments in CFs. Scale bars =200 µm. (D–F) Results from flow cytometry showing expression of adenovirus-mediated expression of target proteins in CFs. (G and H) After stretch, CFs were harvested and RNA was isolated, expression of Ao was determined. Results are expressed as means ± SEM from five independent experiments. NS, No stretch; ST, Stretch.