Fig. 1. Δlhs1Δire1 double mutant cells exhibit a severe growth defect. JTY21 (IRE1/ire1::KanMX4, LHS1/lhs1::TRP1) diploids carrying pRC43 (2µ, URA3, LHS1) were sporulated and tetrads dissected. Four plasmid-containing haploids from a single tetrad, with the genotypes as indicated, were then streaked onto minimal medium containing 5-FOA and incubated at 30°C to select for those cells that have lost pRC43 by spontaneous mis-segregation.