Fig. 6. Expression of NSP4 blocks targeting of VSV-G to the plasma membrane. Cos-7 cells were transiently transfected with a construct encoding NSP4 (D–F) or NSP4Δ140–175 (G–I) and grown for 36 h. Cells were then re-transfected with in vitro synthesized mRNA encoding VSV-G and grown for a further 10 h. Cells were fixed, permeabilized, and double-labelled with a polyclonal antibody against VSV-G (B, E and H) and anti-NSP4 mAb (D and G). As a control, Cos-7 cells were transfected first with a construct encoding GFP (A–C) prior to transfection of VSV-G mRNA. The scale bar represents 10 µm.