Fig. 8. Distribution of ERGIC53, β-COP and Sec13p in cells expressing NSP4. Cos-7 cells were transiently transfected with pcNSP4 (B–D, F–H and J–L) or mock transfected (A, E and I) and grown for 48 h. Cells were fixed, permeabilized and double-labelled with a polyclonal antibody against NSP4 and a monoclonal antibody against either ERGIC53 (A–D), β-COP (E–H) or Sec13p (I–L) prior to analysis by confocal laser scanning microscopy. The scale bar represents 10 µm.