Figure 4. Poxvirus and coronavirus mutants lacking 2′-O methylation are more sensitive to the antiviral effects of murine IFIT-2.
a–d, Studies with VACV. a, Viral replication of VACV-WT or VACV-J3-K175R in wild-type or Ifnar1−/− Mφ (a) or 3T3 MEF expressing GFP (b–d), ISG20 (b), Ifit2 (c) or Ifit1 (d). e, Viral replication of MHV-WT or MHV-D130A in 3T3 cells expressing GFP or IFIT-2. f, Viral replication of EMCV in 3T3 cells expressing GFP or IFIT-2. Error bars, s.d.; dashed line, limit of sensitivity of the assay.