Table 1.
Sequences of primer pairs and probes used for real-time RT-PCR
| Gene | Fragment | |
|---|---|---|
| GenBank Accession # | Sequence 5'-3' | size (bp) |
| BDNF (X55573) | 110 | |
| Forward primer | TGCAGGGGCATAGACAAAAGG | |
| Reverse primer | CTTATGAATCGCCAGCCAATTCTC | |
| Taqman Probe | ACTGGAACTCGCAATGCCGAACTACCCA | |
| NT4 (NM_198190) | 95 | |
| Forward primer | AGCGTTGCCTAGGAATACAGC | |
| Reverse primer | GGTCATGTTGGATGGGAGGTATC | |
| Taqman Probe | TGAGCAGTGAACCCGACCACCCAGG | |
| TrkB (X17647) | 86 | |
| Forward primer | AAGGACTTTCATCGGGAAGCTG | |
| Reverse primer | TCGCCCTCCACACAGACAC | |
| Taqman Probe | CCAACCTCCAGCACGAGCACATTGTCAA | |
| 18S rRNA (X00686) | 76 | |
| Forward primer | CAGGATTGACAGATTGATAGCTCTTTC | |
| Reverse primer | ATCGCTCCACCAACTAAGAACG | |
| Taqman Probe | CCATGCACCACCACCCACGGAATCG | |
| GAPDH (NM_008084) | 130 | |
| Forward primer | AATGTGTCCGTCGTGGATCTG | |
| Reverse primer | CAACCTGGTCCTCAGTGTAGC | |
| Taqman Probe | CGTGCCGCCTGGAGAAACCTGCC | |
| β-Actin (NM_007393) | 144 | |
| Forward primer | CTGGGACGACATGGAGAAGATC | |
| Reverse primer | GTCTCAAACATGATCTGGGTCATC | |
| Taqman Probe | ACCTTCTACAATGAGCTGCGTGTGGCC |