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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Pediatr Gastroenterol Nutr. 2011 Mar;52(3):307–313. doi: 10.1097/MPG.0b013e3181eea177

Figure 2.

Figure 2

Real-time PCR detection of myosin Vb mRNA and immunohistochemical labeling of myosin Vb. A) RNA was extracted from small intestinal biopsies of MVID patient 8 and 9 (designated P8 and P9) as described in Materials and methods, and relative myosin Vb mRNA levels was determined with real-time PCR. B) small intestinal biopsies of MVID patient 8 and 9 and age-matched controls were labeled with antibodies against human myosin Vb. Negative (non-immune first antibody) control staining are shown. The accumulation of myosin Vb in the apical cytoplasm in control enterocytes (arrows) is lost in MVID enterocytes.