Fig. 6. Expression of various loci in cells lacking NHP6A and NHP6B. Northern blot analysis of a MATα Δnhp6a Δnhp6b mutant strain [Δ (MATα); RJY6012] and its isogenic wild-type counterpart [wt (MATα); SEY6210] plus a MATa Δnhp6a Δnhp6b mutant strain [Δ (MATa); DY150] and its isogenic wild-type counterpart [wt (MATa); DY2382]. Ten micrograms of total RNA isolated from cells grown in minimal medium were electrophoresed in a 1.5% formaldehyde–agarose gel, blotted and hybridized with ³²P-labeled probes for the different genes. An ethidium bromide staining of the gel is shown as loading control.