Fig. 2. Release of fMet-Ile peptide from fMet-Ile-tRNA₂^Ile on the ribosome in the presence of 1.2 µM RF [filled circles, RF2(ceAla246); open circles, RF2(oeAla246); filled triangles, RF2(ceThr246); diamonds, RF2(oeThr246)] and in the absence of RF (open triangles) (see the insert for the last two). (A) The fraction of the peptide released from the ribosomal termination complex plotted as a function of time. (B) Natural logarithm of the fraction of peptidyl-tRNA remaining on the ribosomal termination complex plotted as a function of time.