Fig. 4. MALDI-MS analysis of a tryptic digest of RF2(ceThr246) (upper) overlaid with a section of a MALDI-MS spectrum of a tryptic digest of RF2(oeThr246) (lower). The figure shows the increase in mass of the tryptic peptide 246–256 (marked with an asterisk) from RF2(ceThr246) as compared with the same peptide from RF2(oeThr246).