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Fig. 5. Hydrolysis of fMet-Ile-tRNA2Ile on the ribosomal complex paused at a UAA stop codon in the presence of 0.8 µM RF. Filled diamonds, RF2(oeAla246Glu252); open diamonds, RF2(oeThr246Glu252). The small insert shows the natural logarithm of the peptidyl-tRNA remaining on the ribosome. The slope gives kc = 0.01/s for RF2(oeThr246Glu252); kc = 0.016/s for RF2(oeAla246Glu252).
