Fig. 4. The EMAPII-like domain confers on plant MetRS a general tRNA binding property. ³²P-labeled in vitro transcribed tRNA^Met(upper panel) or tRNA^Asp (lower panel) was incubated with native (MOs) or C-terminally truncated (MOsΔC) plant MetRS at different concentrations (0–2000 nM). After electrophoresis at 4°C on a 6% native polyacrylamide gel, the mobility shift of tRNA was visualized by autoradiography.