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Fig. 6. The removal of the EMAPII-like domain affects KM but not kcat in the aminoacylation reaction. The tRNA saturation kinetics in the tRNAMet aminoacylation reaction was determined with pure in vitro transcribed tRNAMet (methionine acceptance of 1150 pmol/A260 unit) in the presence of 2.5 nM MetRS-Os (MOs) or MetRSΔC-Os (MOsΔC). Experimental values (closed symbols) were fitted to the Michaelis–Menten equation (curves).
