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Fig. 7. Salt dependency of the aminoacylation reaction. Initial velocity in the in vitro transcribed tRNAMet aminoacylation reaction was determined for MetRS-Os (MOs) and MetRSΔC-Os (MOsΔC) as a function of KCl concentration in the assay. The results are plotted as percentage of maximum activity obtained at 35 and 90 mM KCl for MetRSΔC-Os and MetRS-Os, respectively.
