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Fig. 9. Time course of aminoacylation of the amino acid acceptor minihelix of tRNAiMet by plant MetRS. Aminoacylation of the amino acid acceptor minihelix of tRNAMet was conducted at 25°C, with 50 µM RNA and 8 µM MetRS-Os (MOs, closed symbols) or MetRSΔC-Os (MOsΔC, open symbols). In one set of experiments, the anticodon microhelix of tRNAiMet was also added at 8 µM in the reaction mixture (squares).
