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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Arch Biochem Biophys. 2010 Dec 24;507(2):332–342. doi: 10.1016/j.abb.2010.12.022

Figure 10. Fluorescence effects on PEP and NPr binding to Trp mutants of EINtr.

Figure 10

Figure 10

(A) PEP titration: Incubation conditions were as described for Fig. 13, except that the final concentrations of all the EINtr proteins used was 82 μg/ml. The baseline fluorescence intensities were: wild-type (filled squares), 42622; W186F (inverted triangles), 41324; W319F (triangles), 37487; W522F (circles), 28078. (B) NPr titration: incubation conditions were as in (A). The baseline fluorescence intensities were: wild-type, 42622; W186F, 72761; W319F, 87741; W522F, 51338.