Table 2.

Distribution of tumor marker immunoreactivity and morphometric values

Tumor Clustera	DCIS	LVI	t-test
E-cadherinb	95±5%	93±6%	p=0.5
Ki-67c	23±4%	25±8%	p=0.5
Size (perimeter)d	1221μ±180	1084μ±160	p=0.1
Shape (elongation ratio)e	0.85±.10	0.70±.05	p=.05

^a100 clusters of DCIS and tumor emboli were individually analyzed for the given immunoreactivity or morphometric measurement and expressed as Mean ± SD. Differences were analyzed with the Student's t-test.

^bE-cadherin immunoreactivity was measured as the percentage of tumor cells exhibiting membrane positivity.

^cKi-67 immunoreactivity was measured as the percentage of tumor cells exhibiting nuclear positivity.

^dSize was measured as the perimeter of the DCIS clusters or lymphovascular tumor emboli and analyzed with ImageJ algorithms.

^eShape was measured as the elongation ratio (ratio of short to long axis) of the DCIS clusters or lymphovascular tumor emboli and analyzed with standard imaging algorithms.